get_filled_border {ddpcr} | R Documentation |
Get border of filled droplets in PNPP experiment
Description
In a PNPP experiment, the rain droplets are the non-empty drops that don't have a
high enough intensity in the positive dimension to be considered as filled
with high quality sample DNA. Only droplets considered as filled are
candidates for the negative
and positive
clusters.
get_filled_border
returns the threshold value in the positive
dimension that is used to determine which drops are filled.
Usage
get_filled_border(plate, well_id)
Arguments
plate |
A ddPCR plate. |
well_id |
Get border of filled droplets for this well. |
Value
Thresholds of filled drops in the positive dimension.
See Also
pnpp_experiment
positive_dim
get_filled_drops
Examples
file <- system.file("sample_data", "small", "analyzed_pnpp.rds", package = "ddpcr")
plate <- load_plate(file)
get_filled_border(plate, "A05")
get_filled_border(plate, "F05")
[Package ddpcr version 1.15.2 Index]