armitage_estimate_sarea {httk}R Documentation

Estimate well surface area

Description

Estimate geometry surface area of plastic in well plate based on well plate format suggested values from Corning. option.plastic == TRUE (default) give nonzero surface area (sarea, m^2) option.bottom == TRUE (default) includes surface area of the bottom of the well in determining sarea. Optionally include user values for working volume (v_working, m^3) and surface area.

Usage

armitage_estimate_sarea(
  tcdata = NA,
  this.well_number = 384,
  this.cell_yield = NA,
  this.v_working = NA
)

Arguments

tcdata

A data table with well_number corresponding to plate format, optionally include v_working, sarea, option.bottom, and option.plastic

this.well_number

For single value, plate format default is 384, used if is.na(tcdata)==TRUE

this.cell_yield

For single value, optionally supply cell_yield, otherwise estimated based on well number

this.v_working

For single value, optionally supply working volume, otherwise estimated based on well number (m^3)

Value

A data table composed of any input data.table tcdata with only the following columns either created or altered by this function:

Column Name Description Units
well_number number of wells on plate
sarea surface area m^2
cell_yield number of cells cells
v_working working (filled) volume of each well uL
v_total total volume of each well uL

Author(s)

Greg Honda

References

Armitage JM, Wania F, Arnot JA (2014). “Application of mass balance models and the chemical activity concept to facilitate the use of in vitro toxicity data for risk assessment.” Environmental science & technology, 48(16), 9770–9779. doi:10.1021/es501955g.

Honda GS, Pearce RG, Pham LL, Setzer RW, Wetmore BA, Sipes NS, Gilbert J, Franz B, Thomas RS, Wambaugh JF (2019). “Using the concordance of in vitro and in vivo data to evaluate extrapolation assumptions.” PloS one, 14(5), e0217564. doi:10.1371/journal.pone.0217564.


[Package httk version 2.6.1 Index]