VST {Seurat}R Documentation

Variance Stabilizing Transformation

Description

Apply variance stabilizing transformation for selection of variable features

Usage

VST(data, margin = 1L, nselect = 2000L, span = 0.3, clip = NULL, ...)

## Default S3 method:
VST(data, margin = 1L, nselect = 2000L, span = 0.3, clip = NULL, ...)

## S3 method for class 'IterableMatrix'
VST(
  data,
  margin = 1L,
  nselect = 2000L,
  span = 0.3,
  clip = NULL,
  verbose = TRUE,
  ...
)

## S3 method for class 'dgCMatrix'
VST(
  data,
  margin = 1L,
  nselect = 2000L,
  span = 0.3,
  clip = NULL,
  verbose = TRUE,
  ...
)

## S3 method for class 'matrix'
VST(data, margin = 1L, nselect = 2000L, span = 0.3, clip = NULL, ...)

Arguments

data

A matrix-like object

margin

Unused

nselect

Number of of features to select

span

the parameter \alpha which controls the degree of smoothing.

clip

Upper bound for values post-standardization; defaults to the square root of the number of cells

...

Arguments passed to other methods

verbose

...

Value

A data frame with the following columns:


[Package Seurat version 5.3.0 Index]