calculate_concentration {ddpcr} | R Documentation |
Calculate template concentration
Description
Calculate template concentration in each wells in a plate using the same formula that QuantaSoft uses. The concentration information is added to the plate's metadata.
Usage
calculate_concentration(plate)
Details
The concentration in a well as number of copies of template per microlitre of sample, and uses the following equation:
(-log(drops_empty / drops_total) * drops_total) / (droplet_volume * drops_total)
Value
A ddPCR plate with the metadata containing a new 'concentration' variable.
[Package ddpcr version 1.15.2 Index]