FRAPdata {FRApp} | R Documentation |
FRAP analysis dataset
Description
The dataset provides 65 measures of fluorescence intensity equally spaced in time over a period of 100 seconds for 132 dendritic spines, for a total of 8580 observations. Data presents a nested hierarchical structure: spines belongs to 54 neurons, grouped in 6 cultures. The number of spines within neurons, and of neurons within cultures is not constant. Experimental condition is applied to 28 neurons (CAP2-shRNA, 71 spines), whereas 26 are controls (SCR, 61 spines). The dataset is a subset of the original version where spines with fluorescence intensity larger than 0.6 at time 0 have been excluded. For a comprehensive description of the data and of the data cleaning steps, please refer to the paper below.
Usage
FRAPdata
Format
FRAPdata
A data frame with 8580 rows and 6 columns:
- Culture.id
Culture id, from 1 to 6
- genetic.id
Experimental condition, SCR for control and SH for CAP2-shRNA
- neuron
Neuron id and experimental condition, nested within cultures
- spine.ID
Spine id, nested within neurons
- time
Time, from 0 to 99.84
- y
Fluorescence intensities
Source
Di Credico, G., Pelucchi, S., Pauli, F. et al. Nonlinear mixed-effects models to analyze actin dynamics in dendritic spines. Sci Rep 15, 5790 (2025). https://doi.org/10.1038/s41598-025-87154-w